mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17354-1-AP (MKNK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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1x10^6 HeLa cells were intracellularly stained with 0.25 ug Mknk2 Recombinant Antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Mknk2 Recombinant Antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Cytometric bead array standard curve ofMP00216-1, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-4-PBS. Detection antibody: 83235-3-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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Cytometric bead array standard curve ofMP00216-2, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-4-PBS. Detection antibody: 83235-1-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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Cytometric bead array standard curve ofMP00216-1, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-4-PBS. Detection antibody: 83235-3-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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Cytometric bead array standard curve ofMP00216-3, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-3-PBS. Detection antibody: 83235-2-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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1x10^6 HeLa cells were intracellularly stained with 0.25 ug Mknk2 Recombinant Antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

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1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Mknk2 Recombinant Antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83235-1-PBS in a different storage buffer formulation.

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Cytometric bead array standard curve ofMP00216-2, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-4-PBS. Detection antibody: 83235-1-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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Cytometric bead array standard curve ofMP00216-3, MKNK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83235-3-PBS. Detection antibody: 83235-2-PBS. Standard: Ag10546. Range: 1.25-40 ng/mL.

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Standard curve of MP00216-1

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Standard curve of MP00216-2

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